BIOSYNTHESIS OF CATALASE IN AEROBICALLY GROWN SACCHAROMYCES CEREVISIAE: EFFECT OF GLUCOSE AND CYCLIC 3′, 5′-ADENOSINE MONOPHOSPHATE
1981 年 27 巻 3 号 p. 209-217
詳細
1981 年 27 巻 3 号 p. 209-217
The development of catalase (EC 1. 11. 1. 6) activity in Saccharomyces cerevisiae was repressed by the presence of glucose during aerobic culture. The catalase activity of glucose-grown cells increased about 4 times when the cells were aerobically incubated with yeast extract for 3hr. Two catalases, A and T, were detected in the glucose-grown cells by polyacrylamide gel electrophoresis, and the content of catalase A increased with increase of catalase activity by the incubation.
The increase of catalase activity was completely inhibited by cycloheximide and not by chloramphenicol. The increase of catalase activity was also markedly repressed by glucose, the repression not being eliminated by 3′, 5′-adenosine monophosphate.
Microbody-like granules were detected cytochemically in the glucose-grown cells, but the increase of catalase content was not closely related to the appearance of microbody-like granules.
