抄録
The L-α-aminoamidase of Brevibacterium A4 is a particle enzyme with a molecular weight of 135, 000; its optimum pH is 9.5 and its optimum temperature 60°. It is probably a metallo-enzyme with an SH group in the active site, and requiring the presence of Mg2+ or Mn2+. The purification tests showed that it hydrolyses a large number of α-aminoamides. The Michaelis constant Km varies little for the different substrates. On the other hand the Vm varies greatly, in particular according to the hydrocarbon chain length.
The use of the α-amidoamidasic activity for the preparation of optically active, natural or non-natural α-aminoacids has been considered.
