STUDIES ON MOLECULAR SIEVE-BOUND INVERTASE AND RE-USE OF SOLID SUPPORT

抄録

Invertase is immobilized on molecular sieve 4A using the metal link method. Crude enzyme preparations gave better recovery of immobilized invertase than partially purified ones, although the number of units bound per g MS was 66% higher with partially purified enzyme. Exposing immobilized enzyme (IME) to high temperatures up to 2hr did not significantly decrease the activity. IME lost 20% activity after storing at room temperature for 2 months. Studies with 6M urea and desorption by ammonium sulfate indicated that the invertase is probably bound to metal activated MS by covalent linkage. MS can be regenerated using a combination of heat and perchloric acid treatments. MS was regenerated and loaded with invertase 5 times and gave identical results with respect to enzyme loading and activity.