The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
ELIMINATION OF RHIZOBIUM INFECTIVITY BY TEMPERATURE TREATMENT
SHIRO HIGASHITOSHIKI UCHIUMIMIKIKO ABE
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1983 Volume 29 Issue 4 Pages 281-285

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Abstract

The infectivity of Rhizobium trifolii 4S (infective strain on white clover) was lost in 50% of the surviving cells when the cells were incubated in broth medium without shaking at 35° for 7 days.
The plasmid DNA extracted from 4S strain and non-infective strains (A1, spontaneous mutant obtained from 4S, and strain H1 and H2 obtained by the incubation of high temperature) were separated by 0.7% agarose gel electrophoresis. Rhizobium trifolii 4S had three plasmids with molecular weights of 210, 280 and 350Md. However, only two plasmids of 280 and 350Md were detected from the non-infective strains A1, H1 and H2.

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