Abstract
1. Further purification of the enzyme responsible for the oxidation of D-glutamic and D-aspartic acids was carried out and an enzyme of 90% purity was obtained.
2. This enzyme could oxidize D-glutamic and D-aspartic acids but was incapable of oxidizing DL-α-aminoadipic and α-aminomalonic acids. The name D-glutamic-aspartic oxidase was designated for this enzyme instead of its previous name, D-monoaminodicarboxylic acid oxidase.
3. The prosthetic group of the enzyme was proved to be FAD.
4. No metallic component was detectable in the enzyme.
5. Some studies concerning the mechanisms of the inhibitory action of KCN are also presented here.
