Abstract
Two fragments from yeast mtDNA were cloned. One fragment had ARS activity and the other was devoid of it. These two fragments were sequenced. The ARS activity in the former was confined within the 151 bp sequence which is a neighbor of the ori/rep sequence. In spite of its ARS activity, this fragment lacked the consensus sequence A/TAAAPy-ATAAAT/A the same as the 186 bp sequence as we previously reported (J. Biochem., 95, 589-592, 1984). In contrast, the latter fragment had a consensus sequence but it lacked ARS activity. In comparing the two sequences, the necessary elements for yeast mitochondrial ARS are considered.
