Abstract
Aspartate and glutamate were formed from glutamine and oxaloacetate with crude enzyme extracts obtained by ammonium sulfate precipitation (35-65% saturation) from the blue-green alga Anabaena cylindrica. In this reaction the 2-amino group of glutamine was transferred to oxaloacetate in the absence of a reluctant. Azaserine, an inhibitor of glutamine -amide transfer, inhibited glutamate formation from glutamine but did not inhibit aspartate formation. Thus, a transamination from glutamine directly to oxaloacetate was indicated. This transamination reaction may be accomplished with glutamine-2-oxoacid transaminase which is found in animal tissues and in yeast.
