MOLECULAR CLONING OF A β-XYLOSIDASE GENE FROM BACILLUS SUBTILIS

Abstract

The β-D-xylosidase gene (xynB) from Bacillus subtilis PAP115 was cloned via the pBR325 vector in Escherichia coli. The resulting hybrid plasmid, named pRH300, contained a 4.6kbp EcoRI-bordered insert. A subcloning procedure, using the pUC8 plasmid, allowed the determination of the location of the xynB gene on a 3.18kbp fragment which was cloned into the pRH271 plasmid (a pBR325 vector harboring the xylanase gene (xynA) from B. subtilis PAP1115). The new vector, named pRH100, enabled E. coli to produce an intracellular xylanase and a cell-bound xylosidase.