PRODUCTION OF LACCASES A AND B BY A MUTANT STRAIN OF TRAMETES VERSICOLOR

Abstract

After induction, the wild type of Trametes versicolor produced two forms of laccase (A and B) in the ratio of 9 to 1. A UV-selected mutant strain of Trametes versicolor isolated on high concentrations of p-xylidine, p-cresol and phenol produced these laccases without induction in the ratio of 4 to 6. After induction with p-xylidine the production of laccase A increased significantly only in the mutant strain. Laccases A and B had catalytic activities proportional to the EPR spectroscopy-detected copper ions present. Several components were determined by electrophoretic analysis.