Abstract
A non-saccharified high stress corn mash system was used to study the acceleration of glycolysis in glucose ethanol fermentation by Saccharomyces cerevisiae. It was found that the controlled addition of ammonium sulfate or ammonium chloride resulted in an accelerated glycolytic rate, independent of the final ethanol yield. Adding high levels of ammonium salt, however, resulted in decreased extracellular pH and delayed cessation of fermentation. The delayed cessation of ethanol production was due to the decrease in extracellular pH, which could be controlled by the addition of citrate buffer.
