Abstract
The acylase of Malbranchea was active against both benzyl and phenoxymethyl penicillin but the former was a poor substrate. The pH, temperature and reaction time optima were 8.0, 37°C and 5hr respectively. Immobilized dry cells retained activity up to 90 days. Cells were maintained for 40 days with good reuse efficiency until the 10th cycle; during this period, the 6-APA level dropped from 0.4 to 0.05mg/ml.
