Abstract
Eight hybridoma cell clones, obtained by fusing murine myeloma cells (P3-NS1/1-Ag-1) with the spleen cells of immunized BALB/c mice, secreted monoclonal antibodies against the 65-kD mosquitocidal protein of the Bacillus thuringiensis strain PG-14 (flagellar serotype 8a: 8b). Of these monoclonal antibodies, six belonged to the IgM class and two belonged to the IgG1 class. In binding tests, they were all specific for the 65-kD protein and did not react to the 25-kD hemolytic protein produced by the same bacterial strain. Indirect competitive enzyme-linked immunosorbent assays with two monoclonal antibodies (IgM), designated D8C1 and D8B4, had a sensitivity of 1ng/ml for the 65-kD protein. There was no competition by the 25-kD protein.
