INFLUENCE ON MINOR PEAKS OF COENZYMES Q OF THE GLUCOSE CONCENTRATION IN THE CULTURE MEDIUM, THE STAGE OF THE GROWTH CYCLE, AND THE DURATION OF THE COENZYME Q EXTRACTION: REQUIRED CONDITIONS FOR DETERMINING THE MINOR COENZYMES Q

Abstract

An HPLC method has been developed to quantify and compare the relative amounts of minor and major coenzymes Q within and between different yeast species and under different cultural conditions. Four control yeast species were studied: Pichia rhodanensis (Q7), Pichia methanolica (Q7 and Q8), Pichia inositovora (Q9), and Rhodotorula glutinis (Q10). The glucose concentration in the culture medium moderately affected the minor peaks of coenzymes Q as did the duration of the coenzyme Q extraction. But the culture age was the main influence on minor and major coenzymes Q. A careful standardization of growth conditions is required to obtain significant and comparative results from one species to another.