Abstract
Four kinds of electrophoresis were used to characterize enzymes in 11 isolates from six species in the genus Pleurotus (Fr.) Quel: Pleurotus ostreatus, P. pulmonarius, P. spodoleucus, P. salmoneostramineus, P. cornucopiae, and P. cystidiosus. They were agarose isoelectric focusing (IEF), polyacrylamide IEF, 7.5% polyacrylamide gel electrophoresis (PAGE) and 4-15% gradient PAGE. Ultimately, the 4-15% polyacrylamide gel-slab electrophoresis was found to be most suitable for our study in numerically analyzing the patterns of seven enzymes. The enzymes were 6-phosphogluconate dehydrogenase (EC 1.1.1.41 6PGDH), malate dehydrogenase (EC 1.1.1.37 MDH), lactate dehydrogenase (EC 1.1.1.27 LDH), glutamate dehydrogenase (EC 1.4.1.4 GDH), glucose-6-phosphate dehydrogenase (EC 1.1.1.49 G6PDH), alcohol dehydrogenase (EC 1.1.1.1 ADH), and esterase (EC 3.1.1.1 Est). The isolates were compared by using an index of similarity (Sm) based on relative mobilities (Rm) of the enzyme bands. By this method, the isolates were reasonably grouped as species. Based on the Sm values, as well as intercompatibilities and morphological characters, the relationships between P. ostreatus, P. spodoleucus and P. pulmonarius were analyzed.
