ANALYSIS OF CHROMOSOMAL DNA OF MUSHROOMS IN GENUS PLEUROTUS BY PULSE-FIELD GEL ELECTROPHORESIS

Abstract

Chromosomal DNAs from four species of genus Pleurotus were analyzed by the clamped homogeneous electric field (CHEF) electrophoresis. The analyses were carried out on protoplasts obtained from monokaryotic mycelia of the following species: P. pulmonarius, P. salmoneostramineus, P. ostreatus and P. cornucopiae. For P. pulmonarius, 6 DNA bands of sizes ranging from 4.9 to 1.6 megabase pairs (Mb) were clearly separated on the gel. For the other three species 6 to 8 bands ranging from 6 to 2 Mb were found. The banding patterns obtained for these four species were found to be different from each other, while those observed for six strains within the same species, P. ostreatus, showed a relatively similar pattern.