IMPROVING TRANSFORMATION METHOD FOR INDUSTRIAL YEASTS: CONSTRUCTION OF ADH1-APT2 GENE AND USING ELECTROPORATION

Abstract

Strong expression system of yeast dominant selection marker gene was constructed for transformation of industrial yeasts. Bacterial aminoglycoside phosphotransferase II (APT2) gene from Escherichia coli transposon Tn903 was inserted between a yeast alcohol dehydrogenase I (ADH1) gene promoter and cytochrome c1 (CYC1) gene terminator. This ADH1-APT2 gene was strongly expressed in the transformed yeasts even in the case of integration type plasmid. Using this strong expressive dominant selection marker gene, five strains in nine industrial yeasts which were tested could not be transformed by lithium acetate method, but these industrial yeasts could be transformed by electroporation.