The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
ISOLATION OF PHAFFIA RHODOZYMA AUXOTROPHIC MUTANTS BY ENRICHMENT METHODS
JOSÉ LUIS ADRIOMANUEL VEIGAJAVIER CASQUEIROMERCEDES LÓPEZCONCEPCIÓN FERNÁNDEZ
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1993 Volume 39 Issue 3 Pages 303-312

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Abstract

To establish the optimal conditions for mutagenesis in the astaxanthin-producing yeast Phaffia rhodozyma when different mutagens are used, dose-response curves for UV radiation, ethyl methanosulfonate and N-methyl-N′-nitro-N-nitrosoguanidine were constructed. An enrichment scheme using the polyene antibiotic nystatin for the isolation of auxotrophic mutants was designed. This treatment resulted in auxotrophic isolates at a frequency of 1×10-3. Uracil-requiring mutants were obtained by 5-fluoro-orotic acids selection at a frequency of 1.67×10-7.

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