DEGRADATION OF XYLAN BY THE XYLANASE SYSTEM OF ASPERGILLUS ORYZAE No. 8
1958 Volume 4 Issue 1 Pages 39-50
Details
1958 Volume 4 Issue 1 Pages 39-50
1) The xylanase system produced by Aspergillus oryzae No. 8 in the wheat bran culture was able to hydrolyze xylan in a degradation limit of 90%.
2) By paper chromatography, the end products from xylan by this enzyme were demonstrated to be xylobiose, xylotriose and xylotetraose.
3) From the observation on the process of xylan digestion by this enzyme it was concluded that this system should be classified into liquefying xylanase or xylanase A.
4) When various xylo-oligosaccharides were used as substrates, it was observed that the higher polymer was hydrolyzed much faster than the lower. The innermost bonds of substrate molecules were most easily hydrolyzed.
5) A main products of xylan degradation by the enzyme system, xylobiose,
xylotriose, xylotetraose and xylopentaose were isolated in a crystalline form or powder by applying the charcoal column technique.
