A GENE ESSENTIAL FOR THE SITE-SPECIFIC EXCISION OF ACTINOPHAGE R4 PROPHAGE GENOME FROM THE CHROMOSOME OF A LYSOGEN

Abstract

An open reading frame (ORF469) was found near the attP core site of the actinophage R4 which encoded a 469-amino acid polypeptide. A gene disruption experiment suggested that the ORF469 gene was essential for site-specific excision of the prophage R4 genome from its host chromosome. The predicted amino acid sequence of the protein showed a similarity to a resolvase of Tn2501 and SpoIVCA of Bacillus subtilis.