Abstract
Diacetoxyscirpenol (DAS)-deacetylating bacteria were isolated from ovine rumen fluid by the ordinary anaerobic roll-tube method in which the energy source substituted glucose, cellobiose and soluble starch for DAS (10ppm) in Medium-10. The isolates were Butyrivibrio fibrisolvens, Lactobacillus sp. and an unidentified bacterium. Among these bacteria, B. fibrisolvens M-14a, which had the highest capability of DAS metabolism, was incubated in a medium containing DAS. The bacteria grew well in the 10 and 50ppm concentrations. At these concentrations of DAS recovery ratios were 7.0 and 20.1%, and 15-acetoxyscirpenol (15-ASP) recovery ratios were 52.8 and 51.2% after 120h of incubation, respectively. In the 100ppm concentration, the bacterial growth was inhibited and the recovery ratio of DAS was 36.1%, and the ratio of 15-ASP was 16.7%.
