TOTAL CROSS SECTION FOR OPTICAL SCATTERING BY SPHERICAL CELLS IN SUSPENSION

Abstract

(1) Absolute values of turbidity in cell suspensions were measured under the narrow beam condition, giving, thereby, correct values of scattering cross section per cell.
(2) By using zero method, average indicies of refraction were obtained for Saccharomyces cerevisiae, Rhodotorula glutinis and Micrococcus luteus.
(3) If cells are assumed as homogeneous spheres, the cross section could be computed by RAYLEIGH-GANS' and HART-MONTROLL'S expressions. The computed values agreed well with the observed ones for the specimen used.
(4) The equations mentioned above will make, accordingly, a convenient theoretical basis for further studies on scattering of light in cell suspensions as will be shown in succeeding papers.