Abstract
As an aid to ecological research of microorganisms, a differentiation of aerobic bacteria, mainly genus Pseudomonas, was studied. On 28 genera, 114 species and 350 strains of type cultures, cultural and biochemical characteristics, such as assimilation of nitrate, ammonium, glutamate, succinate, glucose, and p-hydroxy-benzoate, reaction to B. C. P. milk, formation of soluble pigments, coloration on nutrient agar slant etc., were tested. The results are shown on Table 3, and the author described a differential key of aerobic bacteria, which illustrated on Table 4. The bacteria belonged to Gram negative group, such as Pseudomonas, Achromobacter, Flavobacterium, Escherichia, Aerobacter etc., are biochemically simple, and would be differentiated by this key. On the other hand, the bacteria of Gram positive, such as Micrococcus, Sarcina, Brevibacterium, Bacillus etc., are nutritionally more complex than that of Gram negative group, and would not easily differentiated by this procedure. Furthermore, some problems of bacterial taxonomy were discussed on the basis of the relationship between bacterial flagellation and biochemical characteristics.
