CHROMATOGRAPHIC ANALYSIS OF NUCLEOTIDIC COMPOUNDS IN BACILLUS SUBTILIS

Abstract

1. Quantitative measurement was made on the free nucleotides in the cells of Bacillus subtilis by subjecting the ³²P-labeled cells to ion-exchange column and paper chromatographic analyses.
2.About 40 nucleotide derivatives were detected on paper chromoatograms, among which the following compounds were identified: AMP, CMP, GMP, UMP, ADP, UDP, UDPG and DPN.
3. The incubation with mitomycin C resulted in an increased concentration of total U.V.-absorbing substances, especially those of CMP, UMP, GMP, UDPAX and an unidentified adenine derivative. TMP, thymine and an unidentified UDP derivative were found to appear only in mitomycin treated cells.