BIOCHEMICAL AND CYTOLOGICAL STUDIES ON THE GROWTH OF NOCARDIA SP.

II. PHOSPHORUS METABOLISM WITH SPECIAL REFERENCE TO THE INCORPORATION OF ₃₂P INTO NUCLEOTIDES AT THE STATIONARY GROWTH PHASE

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Phosphorus metabolism of Nocardia sp. CF 222 was investigated using ³²P as a tracer. It was found that the rate of phosphate incorporation into the cells was higher at the logarithmic phase than at the stationary phase. A large amount of phosphorus compounds was released at later stage of logarithmic growth, and 65% (in terms of phosphorus) of them were released in a form of inorganic phosphate. In parallel with the release of phosphorous compounds into the medium, there occurred a decrease of acid soluble organic phosphorus compounds in the cells.
Columnchromatographic analysis showed that the cells at different phases of growth in a nutrient broth had essentially the same patterns of nucleotides and related compounds. However, some changes in the pattern were observed when the cells were grown in different media or when the cells grown in nutrient broth for 35hr were transferred to a fresh synthetic medium deficient in phosphate.