1997 年 81 巻 p. 273-279
In this study, the most favorable kind of culture solution for growth of Parmotrema tinctorum (Nyl.) Hale was 1/100 strength BBM solution (Deason and Bold 1960). Addition of vitamins (thiamine, biotin, and cyanocobalamin), plant growth regulators (IAA and kinetin), or amino acids (glycine, L-glutamine, and L-aspartic acid) did not promote the growth of this lichen, in fact vitamins and IAA inhibited its growth. However, addition of L-asparagine tended to promote its growth. The thallus area increased by 30-40% in 24 days when the lichen was soaked in 1/100 strength BBM solution containing 10 mg/l of L-asparagine for 90 min every 4 days and grown at 100% relative humidity at 20℃ in a growth cabinet illuminated with about 50 μE/m2/sec for 16 hr daily.