The Development and Collagen Ingredients Analysis of Submandibular Gland Acellular Matrix

Abstract

To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients. METHODS: The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry and ultra structure observed. RESULTS: Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy and The collagen fibers showed different diameter and directions and formed complicated network structure and SEM. I, II and III type collagen proteins of submandibular gland before and after cell-extracted were positive under immunohistochemistry. CONCLUSIONS: The main ingredients of submandibular gland acellular matrix were I, II and III type collagen proteins.