Expression and Significance of Apoptosis-Related Factors of Mitochondrial Pathway in Different Phases of Infantile Hemangioma

抄録

It is known that the progression of dermal hemangioma is highly related with apoptosis of vascular endothelial cell. Researches more and more attached importance to study some apoptosis related factors on affacting the proliferation and involution of dermal hemangioma. The bcl-2 family is a group of evolutionary conserved pro- and antiapoptotic proteins that play a pivotal role in the regulation of the mitochondrial-mediated apoptotic pathway. The expression of bcl-2 family members in hemangioma is controversial. Some think that the expression of bcl-2 in hemangioma is inherent and different. The other consider that skin hemangioma do not express bcl-2. In order to illustrate the apoptotic mechanism of dermal hemangioma through the mitochondrail pathway, immunohistochemistry method and in situ hybridization were used to detect the expression of bax, bcl-2,bcl-xl and caspase9 mRNA in hemangioma, including proliferative hemangioma, involutional hemangioma and normal skin tissue. The expression of bcl-2 and bcl-xl in proliferating hemangioma were markedly stronger than that in involuting hemangioma (P‹0.01).On the contrary, the expression of bax and caspase9 mRNA in involuting hemangioma were significantly higher than that in proliferating hemangioma (P‹0.01, P‹0.05, respectively).There were no positive expression of these apoptotic related factors in normal skin tissue.