Dextrin Promotes Proliferation of Cultured MC3T3-E1 Mouse Osteoblast-like Cells and Their Alkaline Phosphatase Activity: Implications for Potential Application of Dextrin as a Binder of Bone Filling Material

抄録

To develop a bone substitute with shape-generating properties, we investigated effects of dextrin, a low viscosity material, on MC3T3-E1 mouse osteoblast-like cells in vitro. At concentrations of 0.1 and 1.0 mM, dextrin promoted proliferative activity of MC3T3-E1 cells, whereas at concentration of 10 mM and higher, it negatively affected cell survival. In an alkaline phosphate (ALP) assay, MC3T3-E1 cells from experimental groups that were exposed to all tested concentrations of dextrin showed significantly higher ALP activity values than cells in control group after day 9 in culture. In addition, blue-violet color in histochemical ALP staining experiments was detected in all groups on day 5 in culture; however, the most intensive staining was observed in MC3T3-E1 cells treated with 0.1 mM dextrin on day 11. Given to previously proven good biocompatibility of dextrin in vitro, our present results suggest that dextrin can be combined with bone filling material as a binder for clinical applications.