2019 年 28 巻 1 号 p. 37-42
Macrophages are involved at an early stage of the inflammatory reaction and have an important role in wound healing. Granulocyte-macrophage colony stimulating factor (GM-CSF) stimulates the proliferation and differentiation of macrophages. We investigated the secretion of tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) from mouse macrophages (RAW264.7) activated by GM-CSF. RAW264.7 cells were cultured on titanium (Ti) discs. Secretion of TNF-α and IL-4 was evaluated using enzyme-linked immunosorbent assay (ELISA) at 24 h and 48 h. Cell morphologies were observed using SEM, and cell viability was accessed by an MTT assay.
GM-CSF caused rough and irregular surface morphology on the macrophages and resulted in a significant difference in cell viability after 48 h (p<0.05). TNF- α secretion significantly decreased after 48 h without GM-CSF compared with that at 24 h (p<0.05). GM-CSF significantly increased the secretion of TNF-α after 24 h and 48 h (p<0.05). IL-4 secretion was significantly different with or without GM-CSF stimulation at 24 h and 48 h (p<0.05). There was a significant increase in IL-4 secretion 24 h and 48 h after GM-CSF stimulation (p<0.05).
These results suggest that macrophage stimulated GM-CSF may promote secretion of anti-inflammatory and pro-inflammatory cytokines on Ti.