2019 年 28 巻 4 号 p. 323-328
To investigate differences in curative effect of bone mesenchymal stem cells grafting via arteries and veins on 6-hour ischemia-reperfusion. A 2-hour middle cerebral artery ischemia-reperfusion model was established in 27 SD female rats. BMSCs were isolated and cultured using the whole bone marrow adherent technique, identified by flow cytometry. The expression of surface antigen CD90, CD29, CD106, CD11b, CD34 and CD45 on BMSCs was 95.7%, 97.3%, 52.7%, 6.01%, 2.95% and 2.26%, respectively. When compared with two PBS groups, the neurological severity was ameliorated significantly and expression of GFAP and Bcl-2 in infarction border zone were further up-regulated in two BMSCs groups. Moreover, the levels of TNF-a in serum and TUNEL staining in brain tissue were significantly reduced after transplantation. BMSC grafting via caudal veins was the best approach during the early periods of ischemia-reperfusion due to minor trauma and the rich source of BMSCs. It provides a possible way to future clinical treatment of cerebral infarction by stem cell infusion to patients.
