Effects of Naringin on the Proliferation and Osteogenic Differentiation of Canine Bone Marrow Stromal Cells In Vitro

抄録

The aim of the present study was to investigate the induction of osteogenic activity and osteogenic differentiation of canine bone marrow stromal cells (BMSCs) by naringin. BMSCs were separated and cultured in vitro and identified by flow cytometry. Then, different concentrations of naringin (1×10^-5, 1×10^-6, 1×10^-7, 1×10^-8, and 1×10^-9 mol/l) were added to BMSCs cultured in DMEM to induce their differentiation. The effects of naringin on BMSCs were evaluated by CCK-8 assay and by measuring the activity of alkaline phosphatase (ALP). Calcium nodules, a marker of osteogenesis, were detected by alizarin red staining. The results of cell-surface marker analysis showed that the cells were negative for CD34 and CD45 expression, with values of 0.126% and 0.075%, respectively, and positive for CD90 expression, a value of 95.4%. Naringin at a concentration of 10^-6 mol/l obviously promoted cell proliferation, among the concentrations, this concentration achieved the best effects on proliferation and osteogenic differentiation. Calcium nodule staining was positive. The findings demonstrate that naringin can induce the differentiation of bone marrow stromal cells into osteoblasts and that naringin at a concentration of 10^-6 mol/l can enhance the proliferation and osteogenic differentiation of BMSCs.