2025 Volume 34 Issue 1 Pages 29-34
In this study, we aimed to investigate the effects of essential amino acid (EAA) deficiency on human gingival fibroblasts (HGFs). EAA deficiency inhibited HGF proliferation and migration, leading to impaired cellular functions. It also caused morphological changes, leading to thinner and less elongated cells, and suppressed collagen gene expression and synthesis. EAA deficiency promoted autophagy, as indicated by the increased expression levels of LC3 and decreased expression levels of p62. Overall, these findings suggest that EAA deficiency significantly impairs wound healing and tissue regeneration by HGFs. Therefore, adequate EAA intake is important to enhance tissue repair, particularly in periodontal treatment, by supporting the cellular functions necessary for healing.
