Laser Surface Treatment of Plastics for a Single-Channel Multiple Immunoassays Chip

Abstract

  A novel micro immunoassay chip for detecting biomarkers in blood plasma has been developed by using an injector-based antibody immobilization. Since multiple detectors were made in a single channel, the chip was able to detect various biomarkers simultaneously. UV Laser processing of the substrate was performed to enhance immobilization of the antibody. In P1CP, which is a biomarker for osteoporosis, this simple plastic chip requires only 1 micro liter per lane of blood plasma and 30 min of reaction time, one twentieth the sample volume required for the ELISA-kit and six times the through put.