ペプチドミクスに基づくバイオマーカーや生理活性ペプチドの発見

抄録

Peptidomics has received more attention in the search for biomarkers, as almost all the potential biomarkers reported through serum or plasma analysis have turned out to be proteolytic fragments of proteins. Peptidomics is also promising as a tool to discover bioactive peptides which are beyond the reach of current proteomics. But there are many problems to be solved in peptidomics. Both generation and degradation pathways of the peptides are present together in the cells, tissue and plasma, and endogenously generated peptides are easily converted into secondary products by proteolysis during extractions and preparations. Proteins are simultaneously digested by proteases, providing numerous peptides. Therefore, it has long been recognized that the real status of endogenous peptides are extremely difficult to analyze and identify. However, recent progress in peptidomics allowed us to identify peptides in their endogenous forms and to apply this technology to the biomarker searches, even though there were still partially digested peptides. On the other hand, peptidomic analysis has not succeeded in the identification of new bioactive peptide in the mammals, but a recent study made it possible by using an improved peptide preparation method. In this symposium, I would like to summarize problems in peptidomics, and then introduce new technologies and recent progress in the discovery of biomarkers and bioactive peptides, which will be detailed by the speakers.