尿プロテオーム解析標準化のためのガイドライン

抄録

HKUPP (Human Kidney and Urine Proteome Project) has been recently approved as one of HUPO-sponsored scientific initiatives on October 2007, in which more than 150 participants collect and share proteomics data on the kidney and urine in order to understand functions of the kidney and mechanisms of disease progression, and finally to discover biomarkers and target molecules for new therapeutics of kidney diseases. Based on our continual discussion through foregoing symposia and workshops held in HUPO Annual Congresses and American Society of Nephrology Renal Weeks, we are planning to draft HKUPP Standards and Guidelines for Urinary Proteome Analysis. These guidelines are really needed for current situation under which rapidly increasing number of investigator are trying or applying urinary proteomics in aims of biomarker discovery. In this presentation, I will outline the current concerns pertaining to urine proteomics with special emphasis on variability of urine proteome, and provide our initial HKUPP Standards and Guidelines for collection, handling, storage and treatment of urine to perform urinary proteome analysis in the more standardized way. Our aim is neither to use these standards and guidelines as the rules and regulations for publication in proteomics journals nor to discourage analysis of the archival urine samples collected for a long time of period. In the standards and guidelines, we keep in mind that they must be: i) generalized for all of most of techniques; ii) generalized for all or most of laboratories and iii) balanced between perfection and practicability.