Abstract
Since 1994, our laboratory has been studying the QUIDEL allergy screen test (QAS) which is based on an enzyme-linked immunosorbent assay and can analyze six types of allergens simultaneously. QAS covers allergens from pollen, house dust, food and so on. In the present study, we attempted to detect serum allergen-specifie IgE antibodies to these pollen allergens. Method: Thirty patients with nasal allergy aged 10-60 years were asked about their history and subsequently underwent several tests including nasal mirror, eosinophils in rihnorrhea, scratch tests, and provocation tests. Allergen sticks of QAS-III (ragweed, Dactylis glomerata, Anthoxanthum odoratum, ceder and mugwort) were used. A 100μl test solution was made to react with a very small amount of serum allergen-specific IgE antibody in a bat (solid phase). Results: Eosinophils in rhinorrhealargely correlated with the coloring of QAS-III. Thus, tests of allergen-specific IgE antibodies by ELISA-QAS can detect various allergens simultaneously. This method is less painful to patients.
