脱細胞技術を用いた喉頭切除後の再生に向けたシーズ開発

抄録

Hemilaryngectomy or total laryngectomy compromise the quality of life of patients with laryngeal cancer, and a regenerative strategy for the surgical defect is warranted. Since the larynx is a composite organ consisting of cartilage, muscle and mucosa, it is difficult to regenerate laryngeal tissues even with the use of currently available scaffolds derived from extracellular matrix (ECM), such as collagen, gelatin or hyaluronic acid. The recently developed decellularization technology allows preparation of a complete scaffold with preservation of the architecture of an organ with preserved ECM. Such bio-scaffolds contain very few surviving cells, therefore, the risk of immune rejection after transplantation is low. Decellularization of the larynx has been attempted using animal larynges with chemical or enzymatic decellularization methods. The important points in the methodology are to effectively eliminate cells, preserve the ECM as much as possible, and maintain the growth factors and vessels to the extent possible. Recellularization with autologous stem cells is possible after decellularization. The decellularization technology may provide a complete bioscaffold for the entire larynx.