喉頭・気管の組織幹細胞研究

抄録

In regard to post-injury tissue regeneration of the larynx and trachea, knowledge regarding the cellular composition of normal tissue and maintenance of its homeostasis, which are fundamental for achieving normalization, remains limited. Cell lineage tracing experiments and organoid fabrication methods have enabled the demonstration of stem cell identities by producing all cell types constituting a tissue from a single cell, while retaining self-renewal capacity. By these two methods, several tissue stem cells of the trachea have been identified, while tissue stem cells of the larynx have not been identified yet.

New technologies have emerged, such as single-cell RNA sequencing for analyzing the genetic information of individual cells and RNAscope for highly sensitive in situ hybridization to visualize cellular localization. These techniques can be employed to explore and validate candidate tissue stem cells. This paper introduces these promising technologies for identifying novel stem cell candidates and outlines the current state of tissue stem cell research related to the larynx and trachea.