鼻粘膜のフィブロネクチンと好酸球の検討
1996 年 89 巻 10 号 p. 1217-1222
詳細
1996 年 89 巻 10 号 p. 1217-1222
The distribution of fibronectin, an extracellular matrix component, was examined in patients with nasal allergy and in non-allergic patients. The distribution and the number of EG2 positive cells in the nasal mucosa was investigated with respect to the clinical effects of anti-allergic agents.
We investigated 11 patients with nasal allergy (5 males and 6 females, age 19 to 57 years) and 8 non allergic patients (6 males and 2 females, age 16 to 72 years). Biopsy specimens were obtained from the inferior turbinate under general or local anesthesia, and then frozen at -70°C. The samples were then sectioned to a thickness of 4 pm using a cryostat. The presence of fibronectin, EG2 positive cells and macrophages was then identified using each monoclonal antibodies and streptavidin-biotin peroxidase complex staining method (DAKO).
In this study, we found that fibronectin was distributed mainly around the basement membrane and vessel walls, and its distribution was similar between patients with nasal allergy and non-allergic patients. The clinical effects of anti-allergic agents correlated well with the number of EG2 positive cells in the superficial layer, but not with the distribution of fibronectin in the nasal mucosa.
