Lectin-Binding Affinities of Laryngeal Epithelial Lesions

Abstract

Twenty-nine laryngeal lesions including 3 hyper-plastic lesions,10 vocal cord polyps,4 dysplastic lesions,6 non-keratinized squamous cell carcinomas, and 6 keratinized squamous cell carcinomas were stained with horseradish peroxidase conjugated lectins, for histochemical studies of the cell membrane.
Both PSA and DBA failed to stain these tissues. WGA, UEA-1, and RCA-1 were demonstrated in all benign lesions, whereas in dysplastic tissues and squamous cell carcinomas, a loss of cell membrane stining was noted in various degrees, especially in non-keratinized squamous cell carcinomas.
None of the dysplastic tissues and squamous cell carcinomas were stained by GSII staining. Some of the benign lesions were slightly stained.