in vitroの肥満細胞の反応性
組織片と浮遊細胞での比較
1987 年 1987 巻 Supplement15 号 p. 40-46
詳細
1987 年 1987 巻 Supplement15 号 p. 40-46
The percentage of histamine release and ultrastructural changes induced by antihuman IgE were studied in chopped tissues and dispersed tissue cells containing free mast cells. Mast cells were dispersed from tonsil and allergic nasal mucosa using pro- teolytic enzymes. Electron microscope examination showed that the morphology of mast cells was preserved. Histamine release of dispersed tissue cells was significantly higher than that of chopped tissues. In addition, dispersed mast cells sometimes showed extensive degranulative changes.
These results demonstrate that histamine release and degranulation of mast cells vary according to different methods used in experiments.
