Determination of the Polyphagous Gene (pph) Locus by Three-point Test with Visible Markers in the Silkworm, Bombyx mori

抄録

In general, the domesticated silkworm, Bombyx mori, is an oligophagous insect that feeds on mulberry leaves. However, one mutant strain, Sawa-J, can feed on other plants and a low cost artificial diet (LP-1) on which ordinary silkworms do not feed. The food preference of Sawa-J was reported to be controlled by the polyphagous gene (pph) belonging to the third linkage group. To determine the pph locus, we performed a three-point test by using Ze and lem genes as markers. We obtained recombination values of 8.64% between Ze and pph, 14.16% between pph and lem, and 20.24% between Ze and lem. Using the value 20.8 between lem and Ze in the silkworm linkage map, the pph locus was mapped to position 12.9 on the third linkage group.