抄録
Ecdysone oxidase (EO) is an enzyme that catalyzes the oxidation of ecdysteroids, the molting steroid hormones in insects. This enzyme may play important roles in the conversion of ecdysteroids to 3-dehydroecdysteroids. In this study, we identified a novel EO, designated bmEO2, from the silkworm, Bombyx mori. A cDNA encoding bmEO2 was cloned by employing the reverse transcriptase-polymerase chain reaction (PCR) technique. The deduced amino acid sequence of bmEO2 showed conserved residues and sequence homology to other known insect EOs and glucose dehydrogenase proteins. Quantitative real-time PCR revealed that the bmEO2 transcript was abundantly present in larval testes of B. mori.
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