Construction of a BAC library and selection of BACs containing orthologs of Bombyx mori genes in Stenopsyche marmorata (Trichoptera: Stenopsychidae)

Toshiaki Fujimoto; Azusa Okumura; Atsuo Yoshido; Yuji Yasukochi; Go Suzuki; Ken Sahara

doi:10.11416/jibs.87.2_061

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Construction of a BAC library and selection of BACs containing orthologs of Bombyx mori genes in Stenopsyche marmorata (Trichoptera: Stenopsychidae)

Toshiaki Fujimoto, Azusa Okumura, Atsuo Yoshido, Yuji Yasukochi, Go Suzuki, Ken Sahara

著者情報

キーワード: bacterial artificial chromosome, caddisfly, high molecular weight genomic DNA, pulsed-field gel electrophoresis

ジャーナルフリー

2018 年 87 巻 2 号 p. 2_061-2_069

DOI https://doi.org/10.11416/jibs.87.2_061

詳細

抄録

We constructed the first trichopteran bacterial artificial chromosome (BAC) library in Stenopsyche marmorata. The BAC library consists of 32,256 clones with an average insert size of 65.38kb. We also made a cDNA library from an S. marmorata adult male and determined 598 unique sequences. Using the obtained sequences we designed putative single tagged site (STS) primers and selected 81 BACs containing orthologs of Bombyx mori genes by three-step PCR selection method. These BACs will be used for gene-based comparative mapping by fluorescence in situ hybridization (FISH) to identify regions of conserved synteny between the trichopteran and lepidopteran genomes.

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