抄録
Silkworms, Bombyx mori larvae, detect phytochemicals in host and nonhost plant leaves via gustatory receptor neurons (GRNs) expressing gustatory receptors (BmGrs). Expression of BmGrs facilitates detection of phytochemicals by GRNs, which is the basis of the molecular mechanism of host selection in silkworms. However, it is unclear which BmGr(s) are expressed in a single GRN. Here, we established a method to isolate a bundle of GRNs in a gustatory sensillum and a single GRN using micromanipulation and laser-capture microdissection to investigate the gustatory receptor expression pattern in a bundle of GRNs and a single GRN in an epipharyngeal sensillum. Our results suggested that several putative sugar- and deterrent BmGrs are expressed in a myo-inositol neuron and a deterrent neuron, respectively, in the epipharyngeal sensillum; these findings provide the basis for future studies of the involvement of BmGrs and GRNs in the mechanism of oligophagy. Moreover, we present evidence that BmGr10, which has been reported to be a myo-inositol receptor, expresses and functions in an epipharyngeal myo-inositol neu-ron.
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