2002 年 71 巻 2 号 p. 79-83
The baculovirus expression vector system is widely utilized as a highly efficient protein production system, which in conjunction with the silkworm, Bombyx mori, is expected to be useful for commercial mass production of proteins. However, efficient mass production using baculovirus vector requires optimization of production conditions including the host condition, virus dose, and harvest time. Using a recombinant baculovirus encoding human serum albumin (HSA), a B. mori cell line (BmN-4) and B. mori larvae, we determined the effect of virus dose on recombinant HSA (rHSA) production and profile. The relationship between the virus dose and rHSA production in vitro (BmN-4 cells) was opposite that in vivo (B. mori larvae). in vitro, rHSA production increased proportionally with the inoculation virus dose and in vivo, rHSA production was inversely related to the virus dose. We also established that virus dose influenced the production profile and determined the optimum harvest time in vitro (72h postinfection) and in vivo (96h postinfection).
