抄録
The baculovirus vector is a widely utilized, highly efficient protein production system, which, with the incorporation of larvae from the silkworm, Bombyx mori, can be used for commercial mass production. Using recombinant baculovirus encoding human serum albumin (HSA) and B. mori larvae, we studied the effects of larval developmental stage and timing of virus infection on recombinant HSA (rHSA) expression. Silkworm larvae were infected with virus on fourth instar day 1 (D1), day 2 (D2), day 3 (D3), or day 4 (D4). Although all cohorts died by postinfection day 4, rHSA expression levels and total soluble protein levels differed between cohorts. The rHSA expression levels decreased from 273μg/larva in cohort D1 to 246μg/larva in cohort D2, to 195μg/larva in cohort D3, and to 138μg/larva in cohort D4. Our results helped identify optimal conditions for rHSA production in silkworm larva using the baculovirus vector.
