Abstract
In order to produce a bioethanol at low cost from cellulosic biomass, it is effective to increase the reduction and recovery of the enzymes. However, saccharifying enzymes such as cellulases, not only adding the enzyme does not act effectively for adsorption to lignin, the recovery rate of the enzyme is also lowered. Therefore, in order to suppress the adsorption to lignin, novel amphiphilic phospholipid polymer in enzyme reaction solution was investigated. As a result, the phospholipid polymer (PMA) that was combined with 2-Methacryloyxyethyl phosphorylcholin (MPC) and methaacylic acid (MA) improved an enzyme saccharification rate of pulverized Japanese cedar powder. Furthermore, phospholipid polymer inhibited the adsorption of enzyme to lignin. The saccharifiaction rate of pulverized Japanese cedar powder was maxim when the concentration of PMA was 0.025 g/g dry mass over. Furthermore, 0.2 (mg/ml) of protein was remained in the saccharification broth.
