P-27 Production of Bioethanol from Pulverized Material of Japanese Cedar by High-temperature Simultaneous Saccharification and Fermentation

Abstract

The present study was concerned with bioethanol production from the pulverized material of Japanese cedar by high-temperature simultaneous saccharification and fermentation (HT-SSF). The pulverized material of Japanese cedar, which was treated with vibration grained named tandem-ring mill, could be efficiently converted into sugar in enzymatic saccarification, In HT-SSF using this pulverized material and high temperature fermentability yeast Schizosaccharomyces japonicus SS4-5 at 40℃, ethanol production was inhibited. To improve the efficiency of ethanol production under high temperature condition, SS4-5 was subjected to UV irradiation. As the result of mutation, we obtained stable ethanol producing mutant, named SS4-5SP, under high temperature condition. In HT-SSF using SS4-5SP at 40℃, ethanol production and ethanol yield were 51.11 g/L and 82.13 %, respectively. High efficiency ethanol production can be achieved by HT-SSF using SS4-5SP.