1990 Volume 54 Issue 5 Pages 114-119
Ophiopogon japonicus Ker.f.nanus hort. was micropropagated on several media and produced regenerated plants. Meristems were cultured on 25 types of media. Basal medium was Murashige and Skoog supplemented with two different plant growth regulators:α-naphtalenacetic acid (NAA), 0, 0.1, 0.5, 1.0 and 2.0mg/l, and 6-benzy laminopurine (BAP), 0, 0.1, 0.5, 1.0 and 2.0mg/l. All media contained 1.0% ager and 30g/l sucrose and were adjusted to pH5.5-5.8 before autoclaving for 20 minutes at 120°C.
The meristems were cultured on MS medium incubated in a chamber in 16hours light at 700lux at 23±1°C and in 8hours darkness. Shoot was in duced in vitro from some meristems on 23 types of media. Calluse was formed on meristems and regenerated shoots from some media containing high NAA concentrations. Root was induced on meristems and regenarated plants from some media containing NAA. But high BAP concentration prevented root formation. Regenerated shoots were transferred to the same medium which allowed shoot growth.
